منابع مشابه
A review of radioimmunoassay for steroids.
This review, based on the current literature, considers the practical aspects of steroid radioimmunoassays. The problems associated with the raising of specific antisera and their characterization are discussed. Features of assay design, reliability criteria and practicability of radioimmunoassays for steroids are considered.
متن کاملDetection of anabolic steroids by radioimmunoassay.
The synthetic anabolic steroids have structural features which distinguish them from the natural steroid hormones. It should therefore be possible to detect the taking of these substances by testing for their presence in either blood or urine. At present suitable techniques include gas chromatography, mass spectrometry and radioimmunoassay. For practical and psychological reasons urine tests ar...
متن کاملRadioimmunoassay of nortestosterone and related steroids.
A radioimmunoassay of nortestosterone and related steroids, including its principal metabolities, is described and evaluated. Antisera against nortestosterone-17 beta-hemisuccinate- and nortestosterone-3-carboxymethyloxime-bovine serum albumin were raised in goats. By using a mixture of such antisera with different selectivity, the cross-reactions of several naturally occurring steroids can be ...
متن کاملSimple radioimmunoassay of five unconjugated ovarian steroids in a single sample of serum or plasma.
A simplified radioimmunoasay procedure is described for determination of free ovarian steroids in plasma or serum. Ether extracts were chromatographed on a Sephadex LH-20 column equilibrated with benzene-methanol (85:15 by vol) and estrone, estradiol, estriol, progesterone, and llcw-hydroxyprogesterone were resolved from each other. With relatively specific antibodies, all five hormones were sp...
متن کامل[Radioimmunoassay of plasma cortisol].
A radioimmunoassay for the determination of plasma cortisol is described. The antiserum against cortisol was produced by the immunization of rabbits with cortisol21-BSA synthesized from cortisol via hemisuccinate derivative. The antiserum obtained had high affinity and high specificity for cortisol. 0.002 ml of plasma diluted with water was used for the assay. After 1,000 dpm of tritiated corti...
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ژورنال
عنوان ژورنال: Folia Endocrinologica Japonica
سال: 1973
ISSN: 0029-0661
DOI: 10.1507/endocrine1927.49.3_629